serp1 protein  (Human Protein Atlas)

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: The association between SERP1 expression and clinical characteristics in SKCM patients.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: SERP1 expression in cancers. ( A ) SERP1 expression in different cancers and normal tissues in TCGA and GTEx pan-cancer data, ns, p ≥ 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001, ( B ) The SERP1 expression in SKCM and normal tissues, ( C ) The volcano plots of DEGs between high and low SERP1 expression groups, ( D ) The heatmap of top 15 up-regulated DEGs, ( E ) The heatmap of top 15 down-regulated DEGs.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Genes related to SERP1. ( A ) The heatmap of top 50 positively RNAs related to SERP1, ( B ) The heatmap of top 50 negative RNAs related to SERP1, ( C ) The PPI network of SERP1 built via STRING, ( D ) The GGI network of SERP1 built via GeneMANIA.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques:

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Functional enrichment analysis of DEGs between high and low expression of SERP1 in SKCM patients. ( A – C ) GO and KEGG pathway enrichment analyses for DEGs between High and -Low expression of SERP1 in SKCM patients. ( D ) The top 10 hub genes ranked by MCC of cytoHubba, ( E ) The top 30 hub genes ranked by MCODE.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Functional Assay, Expressing

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Genetic alteration and protein localization of SERP1 in SKCM patients. ( A ) Bar chart of SERP1 mutation in pan-cancers based on TCGA database. ( B ) SERP1 gene expression and mutation analysis in SKCM. ( C ) The distribution of SERP1 genomic alterations in SKCM. ( D ) The graph of the correlation between SERP1 expression and copy number alterations in SKCM. ( E ) Kaplan-Meier curve of OS in SKCM patients with altered (red) and unaltered (blue) mRNA expression of the SERP1 gene. ( F ) The representative IHC staining images from HPA database presents SERP1 expression in normal and tumor tissues.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Mutagenesis, Gene Expression, Expressing, Immunohistochemistry

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Correlations between SERP1 and prognosis in SKCM patients. ( A ) OS Kaplan-Meier curve for SERP1 in SKCM patients, ( B ) DSS Kaplan-Meier curve, ( C ) PFI survival Kaplan-Meier curve, ( D – O ) OS Kaplan-Meier curve of statistically significant subgroups for ( D ) Female, ( E ) male, ( F ) Age≤60, ( G ) Race white, ( H ) T stage (T2-T4), ( I ) N stage (N1-N3), ( J ) M stage (M0), ( K ) Pathologic Stage (Stage II-IV), ( L ) Radiation therapy No, ( M ) Tumor tissue site Trunk, ( N ) Melanoma ulceration Yes, ( O ) Melanoma Clark Level (Stage II-V).

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques:

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Forest plots of different clinical variables for SERP1 in SKCM patients. Forest plot of different clinical variables on OS ( A ) and DSS ( B ) by multivariate cox regression analysis.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques:

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: The prognostic nomogram for predicting OS and DSS probability. ( A ) The prognostic nomogram for predicting OS probability by the multivariable Cox regression model via the four statistically significant predictors, such as SERP1, N stage, Melanoma ulceration and Breslow depth. ( B ) The prognostic nomogram for predicting DSS probability. ( C ) The time-dependent ROC curves of OS for 1, 3, 5 year. ( D ) The time-dependent ROC curves of DSS for 1, 3, 5 year. ( E ) The calibration curve of OS for 1, 3, 5 year. ( F ) The calibration curve of DSS for 1, 3, 5 year.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques:

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: SERP1 expression is associated with different clinical variables in SKCM patients. ( A ) T classification, ( B ) Pathologic stage, ( C ) Radiation therapy, ( D ) Breslow depth, ( E ) Melanoma ulceration.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Associations of SERP1 expression and immune infiltration level in SKCM patients. ( A ) Correlation of SERP1 expression with immune infiltration level of 24 immune cell types by Spearman’s analysis. ( B ) Twenty-four types of immune cells are plotted according to different SERP1 expression levels.* p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Relationship of SERP1 expression with immune cell level in SKCM. ( A – P ) SERP1 expression showed significant positive related to infiltrating levels of T helper cells, Tcm, Tgd, Th2 cells, Macrophages, Th1 cells, B cells, aDC, T cells, CD8 T cells, Eosinophils and significant negative related to infiltrating levels of NK CD56 bright cells, NK cells, Mast cells, pDC and Th17 cells.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Correlation analysis between SERP1 and relate gene markers of immune cells in SKCM.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques: Biomarker Discovery

Journal: Aging (Albany NY)

Article Title: Low expression of endoplasmic reticulum stress-related gene SERP1 is associated with poor prognosis and immune infiltration in skin cutaneous melanoma

doi: 10.18632/aging.203594

Figure Lengend Snippet: Correlation analysis between SERP1 and immune checkpoints in SKCM.

Article Snippet: Location and qualitative data of SERP1 protein in SKCM and normal tissues were evaluated by the Human Protein Atlas ( https://www.proteinatlas.org/ ) [ ].

Techniques:

Journal: Viruses

Article Title: A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

doi: 10.3390/v11090787

Figure Lengend Snippet: Figure 1. Protein–protein interactions between stress-associated endoplasmic reticulum protein 1 (SERP1) and nonstructural protein (NS)4B. (A) NS4B interacts with SERP1, as shown in a membrane-base

Article Snippet: To further measure the protein level of SERP1 in the DENV-2 infected cells, we failed to detect the SERP1 protein expression in virus infected cells by either in-house mouse, rabbit, or commercial anti-SERP1 antibodies (Genetex, Abcam, Proteintech Group, Atlas antibodies), possibly because of the poor antigenicity of SERP1 (data not shown).

Techniques: Protein-Protein interactions, Membrane

Journal: Viruses

Article Title: A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

doi: 10.3390/v11090787

Figure Lengend Snippet: Figure 2. SERP1 expressions were induced in Huh7.5 cells upon DENV-2 infection and wild-type (WT) replicon transfection. (A) The Huh7.5 cells were uninfected or infected with DENV-2 at an MOI = 1. The quantification of SERP1 transcripts was performed by RT-qPCR at 0, 1, 2, 3, 4, and 5 d.p.i. The relative quantitative values of the SERP1 gene were normalized to the level of β-actin. *, p < 0.05; ***, p < 0.001 (Student’s t-test). (B) Schematic representation of the DNA-launched DENV-2 reporter replicon pCMV-DV2Rep, which was used in a transient replicon assay. The transcriptional expression of the replicon RNA is under the control of the CMVmin promoter, and the 3′ terminus of the transcript is processed by hepatitis delta virus (HDV) ribozyme sequences. The N-terminal 102 amino acids of the C protein (C102), the Renilla luciferase gene (Rluc), the FMDV2A cleavage site, a neomycin resistance gene (Neo), an internal ribosome entry site (IRES) element, the C-terminal 24 amino acids of E (E24),

Article Snippet: To further measure the protein level of SERP1 in the DENV-2 infected cells, we failed to detect the SERP1 protein expression in virus infected cells by either in-house mouse, rabbit, or commercial anti-SERP1 antibodies (Genetex, Abcam, Proteintech Group, Atlas antibodies), possibly because of the poor antigenicity of SERP1 (data not shown).

Techniques: Infection, Transfection, Quantitative RT-PCR, Expressing, Control, Virus, Luciferase

Journal: Viruses

Article Title: A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

doi: 10.3390/v11090787

Figure Lengend Snippet: Figure 3. The SERP1 overexpression inhibited DENV-2 infection, and SERP1 knockdown increased DENV-2 infection. (A) Western blot analysis of Flag-tagged SERP1 proteins in Huh7.5 cells expressing

Article Snippet: To further measure the protein level of SERP1 in the DENV-2 infected cells, we failed to detect the SERP1 protein expression in virus infected cells by either in-house mouse, rabbit, or commercial anti-SERP1 antibodies (Genetex, Abcam, Proteintech Group, Atlas antibodies), possibly because of the poor antigenicity of SERP1 (data not shown).

Techniques: Over Expression, Infection, Knockdown, Western Blot, Expressing

Journal: Viruses

Article Title: A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

doi: 10.3390/v11090787

Figure Lengend Snippet: Figure 4. Knockout of SERP1 by the II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system in Huh7.5 cells decreased SERP1 mRNA levels, and viral yields were significantly enhanced in the SERP1 knockout cells. (A) SERP1 RNA expression patterns in the SERP1 knockout sublines. The products of RT-PCR performed on RNA isolated from the parental Huh7.5 cells (SERP1+/+) and SERP1 knockout sublines (SERP1+/−and SERP1−/−) using the primers in SERP1 exon 1 and exon 3, which generate a 697 bp product. The SERP1 mutant allele was amplified as a 563 bp product, where the SERP1 exon 1 region was deleted. (B) Sequencing analysis of the parental Huh7.5 cells and SERP1 knockout cell lines from the RT-PCR product. (C) Detection of SERP1 mRNA levels in the parental Huh7.5 cells and SERP1 knockout sublines by qRT-PCR. The expression values are normalized to β-actin expression. ***, p < 0.001 (Student’s t-test). (D) Kinetics of DENV-2 replication in the parental cells and SERP1 knockout sublines. The parental Huh7.5 cells and SERP1 knockout sublines were infected with DENV-2 (MOI = 1) at the indicated times. Infectious virus yield in the BHK21 clone 15 cells was quantified by plaque assay. The differences in virus yields between the parental cells (SERP1+/+) and SERP1 knockout cells (SERP1−/−) at 2 or 3 d.p.i. were analyzed using Student’s t-test. *, p < 0.05.

Article Snippet: To further measure the protein level of SERP1 in the DENV-2 infected cells, we failed to detect the SERP1 protein expression in virus infected cells by either in-house mouse, rabbit, or commercial anti-SERP1 antibodies (Genetex, Abcam, Proteintech Group, Atlas antibodies), possibly because of the poor antigenicity of SERP1 (data not shown).

Techniques: Knock-Out, CRISPR, RNA Expression, Reverse Transcription Polymerase Chain Reaction, Isolation, Mutagenesis, Sequencing, Quantitative RT-PCR, Expressing, Infection, Virus, Plaque Assay

Journal: Viruses

Article Title: A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

doi: 10.3390/v11090787

Figure Lengend Snippet: Figure 6. Overexpression of NS4B improves the virus replication in the Huh7.5 cells overexpressing SERP1. (A) Schematic diagram of HA C-terminal fusion constructs of NS2B and NS4B. The N-terminal 2K signal peptide was deleted (△2K-NS4B-HA). (B) Immunoblot analysis of HA-tagged NS2B and NS4B proteins in the parental cells and SERP1-overexpressing Huh7.5 cells. All of the fragments represented in panel (A) were cloned in pLKO-AS2 to tag the C-terminal end of each protein. Equal amounts of lysates were incubated with anti-HA magnetic beads, and the precipitates were analyzed by Western blot using an anti-HA antibody. The parental cells (C) and SERP1-overexpressing Huh7.5 cells (D) were co-transfected with WT replicon and plasmids encoding individual viral proteins, as indicated. The cell lysates were harvested 24, 48, 72, and 96 h after transfection, and the luciferase activity of the transfected cells was measured. The replication efficiency was calculated by determining the ratio of luciferase activity obtained at 48, 72, and 96 h, to the average value obtained from all of the replicon constructs at 24 h post-transfection. The error bars represent the standard errors of the means (SEMs) from three independent experiments. *, p < 0.05; ***, p < 0.001 (Student’s t-test).

Article Snippet: To further measure the protein level of SERP1 in the DENV-2 infected cells, we failed to detect the SERP1 protein expression in virus infected cells by either in-house mouse, rabbit, or commercial anti-SERP1 antibodies (Genetex, Abcam, Proteintech Group, Atlas antibodies), possibly because of the poor antigenicity of SERP1 (data not shown).

Techniques: Over Expression, Virus, Construct, Western Blot, Clone Assay, Incubation, Magnetic Beads, Transfection, Luciferase, Activity Assay

Journal: Viruses

Article Title: A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

doi: 10.3390/v11090787

Figure Lengend Snippet: Figure 7. Hypothetical model of SERP1-mediated DENV-2 infection. (A) SERP1 overexpression inhibits DENV-2 viral RNA replication and titers. (B) DENV-2 NS4B interacts with SERP1. DENV-2 NS4B may alleviate the inhibitory effect of SERP1 on DENV-2 viral replication via the interaction of NS4B with SERP1.

Article Snippet: To further measure the protein level of SERP1 in the DENV-2 infected cells, we failed to detect the SERP1 protein expression in virus infected cells by either in-house mouse, rabbit, or commercial anti-SERP1 antibodies (Genetex, Abcam, Proteintech Group, Atlas antibodies), possibly because of the poor antigenicity of SERP1 (data not shown).

Techniques: Infection, Over Expression